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Sunday, July 19, 2020 | History

2 edition of Phagocytic killing of staphylococci. found in the catalog.

Phagocytic killing of staphylococci.

Deboye Oriade Kolawole

Phagocytic killing of staphylococci.

by Deboye Oriade Kolawole

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Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.)- University of Birmingham, Dept of Microbiology, 1979.

ID Numbers
Open LibraryOL20230958M

Role ofPeroxide in Phagocytic Killing ofPneumococci JANE PITT AND HARRIET P. BERNHEIMER Department ofPediatrics, staphylococci but not enterococci. RESULTS Characterization of mutant strains. The Hdeficient strains, Ml and M2, had been stable in liquid medium for more than a :// Defensins Impair Phagocytic Killing by Neutrophils in Biomaterial-Related Infection S. S. KAPLAN,1,2* R. P. HEINE,3 AND R. L. SIMMONS2 Departments of Pathology1 and Surgery,2 University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania , and Department of Obstetrics, Gynecology, and Reproductive Sciences,

  Phagocytic killing. Bacteria were preopsonized in serum. To allow for substantial phagocytic killing by control cells the pneumococci were incubated in 50% serum for 15 min, while the corresponding figures for streptococci and staphylococci were 25% serum and 5 min and 50% and 3 min respectively, as found in earlier :// With S. aureus pulmonary antibacterial defenses were suppressed at NO/sub 2/ levels of ppm and greater. Exposure to ppm enhanced the intrapulmonary killing of P. mirabilis which correlated with an increase in the phagocytic cell populations lavaged from the lungs; at ppm bactericidal activity against P. mirabilis was ://

RAPID AUTOMATED PHAGOCYTOSIS ASSAY MATERIALSANDMETHODS S. aureus Wood 46 was cultured for 6 h in tryptic soy broth. One and a half milliliters of bacterial culture was centrifuged, and pelleted cells were resuspended in 1 ml of NaClandwereincubatedfor 30minat 37°Cin anEppendorf thermomixer (Eppendorf Inc., Hamburg, Germany) wul of a ,uM stock solution of bis   Clinical Immunology Newsletter Vol. 6, No. 4 April Influence of Antibiotics on Phagocytic Defense Et~l Veringa, M.D. and Jan Verhoef, M.D., Ph.D. Laboratory of Microbiology Faculty of Medicine of the State University of Utrecht The Netherlands During antimicrobial treatment, an important interaction must occur be- tween the drug administered and the host's defenses, in order to eradicate


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Phagocytic killing of staphylococci by Deboye Oriade Kolawole Download PDF EPUB FB2

Neutrophils are essential for host defense against Staphylococcus aureus infections. Although significant progress has been made, our understanding of neutrophil interactions with S.

aureus remains incomplete. To provide a more comprehensive view of this process, we investigated phagocytosis and killing of S. aureus by human neutrophils using varied assay conditions in :// Phagocytosis and intracellular killing of Staphylococcus aureus by bovine blood polymorphonuclear leukocytes after migration in vitro.

Niemiałtowski M, Targowski SP. Phagocytic activity and intracellular killing of opsonized staphylococci (Smith strain) by bovine blood polymorphonuclear leukocytes (PMNL) was studied after their migration in   When treated with mAb 3B3, staphylococci appeared to be internalized by mouse neutrophils (Fig.

5 G). Mouse blood was infected with S. aureus and analyzed for CFU after 30 and 60 min incubation. Compared with mock control, mAb 3B3 promoted phagocytic killing of staphylococci.

As expected, OPK was blocked by pretreatment with CD (Fig. 5 H). Phagocytosis by human polymorphonuclear leukocytes (PMNs) is an important host defense Phagocytic killing of staphylococci. book infections caused by Staphylococcus aureus. Using an in vitro assay, we compared the opsonic requirements for phagocytic killing of prototype strains of encapsulated (type 1) and microencapsulated (type 5 and type 8) S.

aureus by human :// The phagocytosis and killing Phagocytic killing of staphylococci. book 3H-thymidine-labelled Staphylococcus aureus by polymorphonuclear leucocytes (PMNs) and monocytes (MNs) obtained from 50 health donors were evaluated.

In addition, extracellular factors that might influence phagocytosis and killing were studied. The method described gave highly reproducible results. No significant difference was observed in the phagocytic and Using an in vitro assay, we compared the opsonic requirements for phagocytic killing of prototype strains of encapsulated (type 1) and microencapsulated (type 5 and type 8) S.

aureus by human :// The hydroxyl radical scavenger thiourea inhibited H2O2-mediated killing of iron-loaded staphylococci as well as luminol-mediated chemiluminescence. These results suggest that alterations in intrinsic iron content increase killing of staphylococci by H2O2, MN, and PMN-derived cytoplasts by a free radical-mediated :// opsonic requirements for phagocytic killing of strains SAl mucoid (capsule type 1), Reynolds (type 5), and Becker (type 8) in the presence and absence ofcapsular antibodies and complement activity.

The highly encapsulated type 1 strain was killed by phagocytic cells after incubation with homologous antiserum. In contrast, the microencapsulated do not have the same phagocytic capacity (attach-Receivedforpublication 3 November ment and ingestion) as neutrophils. According to Steigbigel et al.

(), the rate of killing once the bacteria had been ingested was similar for both leucocyte populations. Peterson et al. (), how-ever, found that the rate of killing by MNs was slower capacities of mononuclear phagocytic cells.

The data obtained by varying the initial level of infection indicate that the number of ingested bacteria may subsequently alter the kinetics of intracellular killing. In vitro maturation of macrophages in culture was also found to exert a pronounced effect on the kinetics ofbacterial death.

A variety When treated with mAb 3B3, staphylococci appeared to be internalized by mouse neutrophils. Mouse blood was infected with S. aureus and analyzed for CFU after 30 and 60 min incubation.

Compared with mock control, mAb 3B3 promoted phagocytic killing of staphylococci. As expected, OPK was blocked by pretreatment with :// ABSTRACT. Staphylococcus aureus is a pathogen that has been associated with nosocomial infections since the preantibiotic era.

Since the introduction of antibiotics in medical practice in the s, methicillin-resistant S. aureus (MRSA) strains have been emerging in various parts of the world. In view of the important role of the phagocytic system in the defense against this bacteria, we ?script=sci_arttext&pid=S Goldman JM, Th'ng KH.

Phagocytic function of leucocytes from patients with acute myeloid and chronic granulocytic leukaemia. Br J Haematol. Sep; 25 (3)– Peterson PK, Verhoef J, Sabath LD, Quie PG. Extracellular and bacterial factors influencing staphylococcal phagocytosis and killing by human polymorphonuclear   Extracellular slime (Ecs) from three strains of Staphylococcus epidermidis was prepared and added to fresh suspensions of polymorphonuclear neutrophils.

Phagocytic ingestion and killing of opsonised and unopsonised S. epidermidis strains was assessed over time using slide preparations stained by the Gram's method and microbiological culture. Both phagocytic ingestion and killing were Over 90% of the staphylococci were phagocyte associated after 30 to 60 min.

Killing rates were on the order of 66% +/- 12% and 80% +/- 7% after 1 and 2 h of incubation, :// Using an in vitro assay, we compared the opsonic requirements for phagocytic killing of prototype strains of encapsulated (type 1) and microencapsulated (type 5 and type 8) S.

aureus by human PMNs. More than 85% of broth-grown, logarithmic-phase type 5 and 8 S. aureus organisms were killed by PMNs incubated with fresh normal human, rabbit, or Host immunity against bacteria typically involves antibodies that recognize the microbial surface and promote phagocytic killing.

Methicillin-resistant Staphylococcus aureus (MRSA) is a frequent cause of lethal bloodstream infection; however, vaccines and antibody therapeutics targeting staphylococcal surface molecules have thus far failed to achieve clinical In addition, extracellular factors that might influence phagocytosis and killing were studied.

The method described gave highly reproducible results. No significant difference was observed in the phagocytic and killing functions of a single donor's PMNs and MNs when studied several times in one day and longitudinally over a period of weeks The phagocytosis and intracellular killing of different coagulasenegative staphylococcal species by polymorphonuclear (PMN) leukocytes from one healthy donor were compared.

The uptake of strains belonging to a given species varied from 60 to 80% with an Phagocytosis and killing of Staphylococci by human polymorphonuclear and mononulear leukocytes Article (PDF Available) in Journal of Clinical Pathology 31(6) July with 59 Reads.

Our studies, however, clearly demonstrate that purified HNP incubated with PMN severely compromised phagocytic killing of staphylococci when used at a concentration of 25 μg/ml for HNP2, a concentration determined by others to be toxic to eukaryotic cells, and at a concentration of 35 μg/ml for HNP1.

Phagocytosis and killing of cefazolin-exposed Staphylococci and Bacilli by mouse peritoneal macrophages. The phagocytic and microbicidal capacity of proteose peptone-induced macrophages from Balb/c mice was assessed in vitro against Staphylococcus aureus and Bacillus subtilis in the presence of a subinhibitory concentration of cefazolin   To investigate whether increased phagocytic activity influenced subsequent bactericidal activity the rates of both the phagocytosis and killing of a standard inoculum of viable Staph.

aureus were tested and compared with the same concentration of viable